Coding

Part:BBa_K2088690:Design

Designed by: Yuxin He   Group: iGEM16_NAU-CHINA   (2016-10-09)


optimized RBS+ mhbD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 371


Design Notes

none


Source

The original gene ,MhbR can be gotten from the bacteria M5a1 using PCR.The sequence of the gene comes from GenBank https://www.ncbi.nlm.nih.gov/nuccore/EU165545.1

References